#progesterone assay kit

[calbiotechinc]

How Progesterone Assay Kits are Revolutionizing Veterinary Reproductive Studies

Reproductive health is a crucial aspect of veterinary science, particularly for livestock and companion animals. Efficient breeding management and early detection of reproductive issues are vital for animal health, productivity, and welfare. Recent advancements in diagnostic tools, particularly progesterone testing, have transformed the way veterinarians and researchers approach these challenges. Among these tools, the introduction of specialized progesterone assay kits has been a game-changer. These kits offer rapid, accurate, and reliable means to assess hormonal levels, significantly enhancing veterinary reproductive studies.

Understanding Progesterone's Role in Animal Reproduction

Progesterone is a key hormone involved in the regulation of reproduction in mammals. It plays a critical role in preparing the uterus for pregnancy, maintaining pregnancy, and signaling ovulation. Monitoring its levels can provide essential insights into an animal's reproductive status, helping veterinarians determine the optimal time for breeding, diagnose reproductive disorders, and evaluate fertility treatments.

Traditionally, hormone testing in animals required labor-intensive procedures and sophisticated laboratory equipment. However, modern testing methods have simplified this process, allowing for quicker and more precise results.

The Importance of Progesterone Testing in Veterinary Science

Monitoring progesterone levels is essential in managing reproduction, especially in species like cattle, sheep, dogs, and horses. It aids in identifying:

Optimal Breeding Times: Accurate timing increases the success rates of artificial insemination or natural mating.

Pregnancy Status: Progesterone levels can help confirm or rule out pregnancy.

Reproductive Disorders: Abnormal hormone levels may indicate issues such as ovarian cysts or luteal insufficiency.

With the growing demand for precision in veterinary diagnostics, tools that offer ease of use and consistent accuracy are highly sought after.

Advancements in Assay Kits for Veterinary Applications

Assay kits designed specifically for veterinary use have revolutionized the way progesterone testing is conducted. These kits allow practitioners to measure hormone levels efficiently, often in a matter of hours, without the need for complex equipment.

A prominent example of this innovation is the enzyme-linked immunosorbent assay (ELISA). This testing method has gained significant traction in veterinary science due to its ability to provide quantitative results with high specificity and sensitivity. By using these assay kits, veterinarians can conduct hormone analysis on-site or in laboratory settings, making the process both cost-effective and time-efficient.

Key Benefits of Using Assay Kits

The implementation of modern progesterone assay (ELISA) kits has introduced several advantages in veterinary reproductive studies:

Accuracy and Precision: These kits provide consistent and reliable results, reducing the likelihood of diagnostic errors.

Time Efficiency: Testing can be completed quickly, enabling timely decision-making in breeding management.

Cost-Effectiveness: Eliminating the need for advanced laboratory setups reduces overall costs, making progesterone testing accessible to a wider range of veterinary practices.

Ease of Use: Simple protocols make these kits user-friendly, even for non-specialist personnel.

Real-World Applications in Veterinary Reproductive Studies

One of the most significant contributions of modern kits is their role in livestock management. For instance, dairy farmers often rely on these tools to determine the best insemination windows for cows, maximizing reproductive success rates. Similarly, in canine breeding, hormone testing is critical to ensure mating occurs at the right time, improving litter outcomes.

These tools are also invaluable in wildlife conservation programs, where monitoring reproductive health is crucial for endangered species breeding initiatives. By assessing hormonal changes, researchers can better understand the reproductive cycles of exotic animals, contributing to more effective conservation strategies.

The Future of Veterinary Diagnostics

The growing adoption of assay kits reflects the broader trend of incorporating advanced diagnostic tools into veterinary practice. Future developments are likely to focus on increasing the portability and automation of these kits, allowing for even more streamlined testing procedures. Moreover, advancements in digital technology may enable integration with data analysis software, providing veterinarians with comprehensive insights into reproductive health trends.

Conclusion

The introduction of specialized progesterone testing tools has brought a paradigm shift to veterinary reproductive studies. By simplifying hormone analysis and enhancing accuracy, these kits empower veterinarians to make informed decisions, improve breeding outcomes, and address reproductive challenges effectively. As technology continues to evolve, these tools are set to play an even greater role in advancing veterinary science and animal welfare worldwide.

[supervirtualsassistants]

First, we should know about the hormonal assay test

To identify metabolic disorders and processes, or "hormone imbalance," a hormonal assay test is carried out. Oestrogen, progesterone, testosterone, and hormones generated by other glands, such as the parathyroid and adrenal glands, can all be detected (assayed) in the blood in addition to male and female sex hormones. A doctor recommends this test to identify the causes of low infertility. The fundamental justification for this test is still a loss of sexual desire in both males and females. Additionally, men with erectile dysfunction and women with irregular or interrupted periods may have a problem with their reproductive system, necessitating this test.

When would a hormonal assay be necessary :

Specific hormones or related hormones may be examined in various therapeutic settings. Hormonal tests are frequently used to diagnose menstruation, menopause, or fertility problems. Suppose there is a possibility of an endocrine issue, which affects the operation of numerous glands in the body, including the pituitary, thyroid, parathyroid, adrenals, ovaries, and testes. In that case, your doctor may ask for specialized hormone testing.

Tests findings explained :

Hormonal assay test price - Specific hormone concentrations in the bloodstream are determined through hormone testing. These findings are contrasted with a reference range of "normal" levels discovered by examining healthy individuals without symptoms. Therefore, the hormone level could be low, normal, or excessive.

A doctor knowledgeable about the intricate relationships between the endocrine system's components and the limitations of biochemical hormone testing must carefully evaluate each of these results. Similar to how a standard test may not rule out an abnormality, a high or low level may not always indicate sickness.

PROCEDURE OF HORMONAL ASSAY TEST :

Adults must have a band wrapped around their arm three to four inches above the collection site, which is a superficial vein in the elbow pit. The needle cap is then removed and held in place with the vein, pulling the skin taut. The required amount of blood sample is then collected by slowly pulling the syringe plunger out of the syringe. The needle is taken out, the wrap band is undone, and gauze is spread over the location of the collection. The blood is then transferred immediately.

For the hormone assay, there is no extra preparation required. Before having a hormone assay:

Have any underlying medical issues?

Have any allergies?

Your doctor will provide particular instructions based on your condition.

FEMALE HORMONAL ASSESSMENTS (TESTS) FOR INFERTILITY :

Female hormonal testing is advised when anomalies are found in your menstrual cycle or during a physical check. In addition to estrogen and progesterone levels, FSH and LH levels, Inhibin B, Anti Mullerian Hormone (AMH), testosterone and other androgens, thyroid tests, and prolactin levels are among the hormonal testing we provide at our clinics in Beverly Hills, Sherman Oaks, and Mexico. The outcomes of these hormonal tests aid in choosing the most appropriate course of treatment for every patient.

DO YOU HAVE A HORMONAL IMBALANCE? HOW TO TELL:

Numerous symptoms, including unexpected weight gain, hair loss, exhaustion, and skin problems, can be caused by an imbalance in hormones. Therefore, you may assume hormones are to blame for your symptoms but are unsure how to determine whether you have a hormonal imbalance. If so, keep reading because you'll discover how to check your hormone levels, including one of the simplest yet most reliable methods: hormone test kits you can use at home. Let's start by going through some of the signs and symptoms that a hormonal imbalance might produce.

Signs of an imbalance in hormones

If you're interested in learning to determine whether you have a hormonal imbalance, one step you can take is being familiar with the potential symptoms of hormone imbalances.

But first, a disclaimer: everyone's symptoms of hormone imbalance will differ, and they frequently rely on things like age, medical history, and more. Making an appointment to talk to your doctor about any symptoms you're having should be a top priority if you don't know why you have them.

The following list of signs and symptoms of hormone imbalance:

- Fatigue or feeling exhausted

- Gaining weight

- A rise in body fat

- Irregular cycles

- Cold sensitivity

- Heat sensitivity

- A hot flash

- Sweats at night

- Depression

- Sleep disruption

- Low-quality drive

- Mental exhaustion

Detecting hormonal imbalances

It's not always the case that a hormone imbalance manifests in such blatant symptoms. It may be difficult for someone, for instance, to determine if their depression is caused by a life experience or by hormone levels negatively influencing their mental health. Because of this, seeking medical advice whenever you have questions about your health is a good idea.

FINAL WORD :

Hormonal assay test price - Understanding a hormonal imbalance can be a fantastic starting point for learning about the relationship between your hormones and your general health and well-being. Take it a step further by using one of our at-home hormone tests to monitor your hormone levels while relaxing in the comfort of your own home.

[ijtsrd]

Effects of Carbegoline and Bromocriptine on Prolactin, Progesterone, Luteinizing and Follicle Stimulating Hormones in Hyperprolactinaemic Infertile Women in Orlu, Nigeria

This was an interventional study which evaluated the effects of bromocriptine and carbegoline on prolactin, progesterone, luteinizing and follicle stimulating hormones in hyperprolactinaemic infertile female subjects in Orlu, South East Nigeria.Hyperprolactinaemic females who were attending Gynaecology Clinic at Imo State University Teaching Hospital IMSUTH Orlu participated in the study. The participants were divided into two groups. Group A 30 hyperprolactinaemic females were administered 1.25mg bromocriptine at night for the first one week 2.5mg at week two then 2.5mg twice daily for eighteen weeks. Group B 30 hyperprolactinaemic females were administered 0.25mg carbegoline twice weekly for eight weeks. Serum prolactin, progesterone, LH and FSH were assayed before and after treatment using enzyme linked immunesorbent assay ELISA kits. The significant difference between the mean values of prolactin, LH, FSH and progesterone, in the pre and post treatment with carbergoline and bromocriptine was determined by paired t test. Independent student t test was used to compare between the two treatments. P value 0.05 was considered as statistically significant. 

by Ajaero, Oluchi Chinwe | Unekwe, Prince Chiazor | Ajaero, Nnaemeka Uchendu "Effects of Carbegoline and Bromocriptine on Prolactin, Progesterone, Luteinizing and Follicle Stimulating Hormones in Hyperprolactinaemic Infertile Women in Orlu, Nigeria" 

Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, 

URL: https://www.ijtsrd.com/papers/ijtsrd45169.pdf 

Paper URL: https://www.ijtsrd.com/medicine/other/45169/effects-of-carbegoline-and-bromocriptine-on-prolactin-progesterone-luteinizing-and-follicle-stimulating-hormones-in-hyperprolactinaemic-infertile-women-in-orlu-nigeria/ajaero-oluchi-chinwe

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[helvaticacare]

AN OVERVIEW OF MONOCLONAL ANTIBODIES AND ITS APPLICATIONS

Antibodies or immunoglobulins (Ig) are glycoproteins generated by differentiated B lymphocytes called plasma cells in response to susceptibility to antigens. The diversity of antibody responses to various target antigens is because of the gene recombination process in the hyper-variable areas of antibodies.

Throughout the recombination technique in their genes, antibodies experience gene rearrangement that enables them for various binding. Antibodies’ tremendous specificity and diversity have made them notable molecules with increased efficiencies in several therapeutic or diagnostic applications. The polyclonal antibody, monoclonal antibody and recombinant secondary antibody, as well as antibody fragments, can be used for western blotting. Polyclonal secondary antibodies are the most common form of secondary antibodies ab 2 fragments in use.

Monoclonal antibodies (mAbs) are antibodies produced by identical clones of B lymphocytes against a particular antigen. mAbs are similar in several properties such as protein sequence, antigen-binding site region, binding affinity for their targets, and identical downstream functional effects.

Monoclonal antibodies (mAbs) are commonly administered via the subcutaneous (SC) route. However, bioavailability is often reduced after SC administration. In addition, the sequential transfer of mAbs through the SC tissue and lymphatic system is not entirely understood.  

According to the immune response’s heavy chain constant region, distribution, and function, Monoclonal antibodies can be classified into different classes or isotypes. Mammalians have five different antibodies, including

IgG,

IgM,

IgD,

IgA, and

IgE.

The IgG is the most appropriate and abundant type from those antibody isotypes because it plays a vital role in the immune response. For this cause, most antibodies used in research, therapy, and diagnostics are IgG immunoglobulins.

THERAPEUTIC AND DIAGNOSTIC APPLICATIONS OF MABS

Monoclonal antibodies are valued reagents for an abundance of applications. Due to their selectivity, specificity, high binding affinity, and immunogenicity/low toxicity, monoclonal antibodies are suitable for clinical, environmental, elemental research and more. They are used in applications of therapeutics and diagnostics, including medical devices, vitro tests, and medical imaging.

Through therapeutic applications, monoclonal antibodies can be used in their naked form or as carriers by being conjugated to a tiny molecule or drug. While performing diagnostic and research applications, monoclonal antibodies are often conjugated with fluorescent tags for visual detection of targets or enzymes.

1.MAbs in Biochemical Analysis

Symptomatic tests based on MAbs are reagents that are routinely used in radioimmunoassay (RIA) and enzyme-linked immunosorbent assays (ELISA) in labs. Those assays estimate the circulating concentrations of hormones like insulin, human chorionic gonadotropin, growth hormone, progesterone, thyroxine, triiodothyronine, thyroid-stimulating hormone, and several other tissue and cell products. In current years, various diagnostic kits using MAbs have grown to be commercially available. Now it is helpful for diagnosis of different diseases:

In Pregnancy: Pregnancy by indirect detection of the urinary levels of human chorionic gonadotropin.

In Cancers: Cancers estimation of plasma carcinoembryonic antigen in colorectal cancer and prostate-specific antigen for prostate cancerous cell. Besides diagnosis, evaluation of tumour markers is also essential for the diagnosis of cancers. A constant fall in specific tumour antigens is observed with a contraction in tumour size following treatment.

In Hormonal disorders: Hormonal dysfunctions analysis of triiodothyronine, thyroxine and thyroid-stimulating hormone for thyroid disorders.

2. Use of MAbs in Therapy against Complications of Viral Infections

Cytomegalovirus (CMV) induces severe immunocompromised illnesses, such as patients with AIDS and those going through organ transplants. Infection frequencies may increase up to 75% in those negative for CMV who receive kidneys from seropositive patients. CMV infection can result in retinitis and gastroenteritis in HIV-infected patients and may also cause chronic pain intrauterine disease.

About 40,000 cases of congenital CMV infection are recorded each year; mental retardation and hearing loss might occur in around 25% of those cases. Presently, there is no vaccine against CMV. Ganciclovir, foscarnet, and (S)-1-[3-hydroxy-(2 phosphonylmethoxy) procytosine are a few of the possible treatments for Cytomegalovirus infection.

Another method of medication is via regulation of anti-CMV hyper immunoglobulin obtained from combined sera of CMV-seropositive persons. Passive immunisation has been attested to reduce the severity of CMV and block mother-to-infant transference.

Furthermore, humanized antibodies may evacuate the virus from infected tissues, and a purpose earlier thought to be exclusive to cytotoxic T lymphocytes. Many doctors use a blend of antiviral agents and immunoglobulins in patients in danger of CMV infection. MAbs may also reduce the number of antiviral agents needed for treatment. MAbs against murine CMV polypeptides are shielding in animal models.

Original Source: https://www.h-h-c.com/an-overview-of-monoclonal-antibodies-and-its-applications/

[juniperpublishers-gjorm]

Outcome of In Vitro Fertilization in Women with Discordant Values of Anti Mullerian Hormone and Antral Follicle Count

Authored by:  Swati Verma*

Introduction

Among various proposed biomarkers, Antral Follicle Count (AFC) and Anti Mullerian Hormone (AMH) are said to be most favorable in predicting ovarian reserve and response to stimulation.Both generally have good correlation with each other and are often used interchangeable. There is a growing evidence of consistent association of these biomarkers with ovarian response, number of retrieved oocytes and live birth rate [1-4]. Nonetheless discordance between the two does occur and has been discussed in recent trials [5,6]. The discordance between AFC and AMH raises a doubt regarding the usefulness of AMH or possibility of some laboratory error [7]. It may also suggest existence of some subtle ovarian pathology, not demonstrated by ultrasonography done for assessment of AFC. A very limited data exits on IVF outcome in women with discordance between AMH and AFC. Therefore we conducted index review among women undergoing In-Vitro Fertilization (IVF) at our center with the following objectives;

a) To define the discordance between AMH and AFC in women undergoing IVF

b) To study the IVF outcome in women showing discordance between AMH and AFC

c) To compare the Metaphase 2 (M2) oocytes rate in women with discordant AMH and AFC in relation to their IVF outcome

Study Design, Materials & Methods

This retrospective analysis was conducted from January 2012 to December 2015 at an infertility centre of North India. There were 1754 women who underwent IVF cycle during this period. After fulfilling inclusion criteria 807 women were found eligible for the study (Figure 1). The women included in the analysis were with their age below 35 years, day two serum follicle stimulating hormone (S.FSH) levels below 10IU/ml, first IVF cycle with self oocytes, received ovarian stimulation with standard GnRH antagonist protocol along with fresh embryo transfer done. Most of the women with polycystic ovaries (PCO) were excluded from the study as fresh embryo transfer was not possible to avoid risk of OHSS. The women with PCO who underwent fresh ET were included in the analysis. Although this was a retrospective analysis and did not involve any active intervention on patients, approval was taken from the institutional review board.

AFC and AMH determination

The ovarian reserve was assessed by AFC, serum AMH and day 2 S.FSH as a routine. Total number of AFC was measured on day 2 of cycle by transvaginal ultrasound scan and all the follicles ranging from 2 to 9 mm were included. AMH measurement was done using the AMH Gen II ELISA kit .The assay kit has a sensitivity of 0.08ng/ml, and intra- and inter-assay coefficients of variation of less than 5.4 and 5.6 respectively.

Ovarian stimulation protocol

Standard GnRH antagonist protocol for ovarian stimulation was followed in all women. Starting dose of injection Gonadotrophins was decided according to the value of AFC, AMH and S. FSH. Ultrasound guided follicular monitoring was done and injection GnRH antagonist. 25mg S/C was added once one or more follicles reaches 13-14mm in size. Dosages of injection Gonadotrophins were adjusted according to ovarian response. Injection urinary hCG 10000 I.U was given as oocyte maturation trigger in the patients who were not at risk of OHSS. Women with more than 12 follicles of 16 mm and serum oestradiol levels more than 3500pg/ml were given modified oocytes maturation trigger with injection decapeptyl 0.2mg subcutaneous. Oocyte retrieval was done 34-36 hours after the maturation trigger. All women received injection progesterone 50 mg intramuscular daily for luteal phase support, started after oocyte retrieval. Women who received modified maturation trigger were administered injection urinary hCG 1500I.U on the day of oocyte retrieval and tablet oestradiol valerate 2mg, 8 hourly along with standard luteal phase support. All women were subjected to embryo transfer with two good quality embryos on day 2 of oocyte retrieval.

Distribution of subjects and finding cut-off values for AMH in each AFC group

Study group were categorized into three groups according to AFC. Group 1: ≤7 (n=196), Group II: 8-15 (n=425), Group III: >15 (n= 186). For each AFC group (≤7, 8-15 and >15), entire range of AMH values were binned at 5 percentile values using SPSS 16. Total 20 intervals of AMH values over whole range of data were obtained. IVF outcome (pregnancy yes and no) over various AMH intervals was plotted as histogram. An interpolation line was drawn to demonstrate the trend using two moving average of Excel. Change of trend in IVF outcomes was noted by observing the graph and corresponding cut off values for AMH were defined.

Statistical analysis

According to the cut off AMH values three subgroups i.e. low discordant, concordant and high discordant were obtained within each AFC group. IVF outcomes in each of three groups were analyzed comparing three sub groups using Pearson Chi Square test and the level of significance was calculated. The twotailed value of P < 0.05 was considered statistically significant.

For each AFC group, AMH cut off values were different according to their respective percentiles (Figure 2-4). Only 20.07% (162/807) women among three AFC groups were observed showing concordance between AMH and AFC. One third of women i.e. 33.7% (272/807) included in the study had higher AMH values (high discordance) whereas 46.22% (373) had lower AMH values (low discordance) than expected according to their AFC (Table 1). The three subgroups within each AFC group were comparable for age, duration and aetiology of infertility and indications for IVF. Within each AFC group women showing concordance between AMH and AFC were associated with better IVF outcomes (Table 2). Pregnancies in women in Group 1 and Group III with both high and low discordant AMH values were comparable (42% Vs 43.9% and 30% Vs 32.6%). Women with AFC 8-15 but either high AMH i.e. Group II with high discordance had lowest pregnancy rate (25%). Although two other AFC Groups i.e.1 and III with high discordant AMH values did not follow the same trend, pregnancy rate was 43.9% and 32.6% and it was higher compared with their respective low AMH subgroup women (Figure 2-3). Highest PR was observes in women of Group 1 with concordance AMH values (59.6%). Women with low discordant AMH values of all three AFC groups were reported with lower (Group I, II and III; 42%, 39% and 30% respectively). Women in Group III with high discordance (this group mainly consisted of PCOS women) had PR 32.6%; indicating that higher AFC with higher AMH also did not have higher conception rate when embryos were transferred in the same cycle.

Mean M2 oocytes rate for pregnancy positive versus negative women among three groups is shown in Figure 5 & 6. In the women with concordant AMH values mean M2 oocytes rate for IVF outcome positive vs. negative were in Group I, II and III were 4.33 Vs 2.98; p value.028, 8.8 vs. 6.8 p value .032 and 10.11vs 9.06; p value .048 respectively. The women of all subgroups with positive IVF outcome demonstrated higher number of M2 rate compared to negative IVF outcome and difference was also statistically significant. The number of M2 oocytes also showed rising pattern corresponding to the rise with AMH values within each AFC category.

Discussion

AMH has emerged as reliable indicator and autonomous marker of ovarian function because of its strong correlation with AFC and operator independency. Earlier there were various controversies regarding AMH values in relation to laboratory assays. But now a well-established assay Gen II EUSA is available to measure AMH; as standard techniques worldwide. Comparable performance of AMH and AFC in IVF treatment has been documented and may be explained to certain extent by the fact that source of AMH is granulosa cells of antral follicles [8,9]. Various studies have described discordance between AMH and S.FSH in women undergoing assisted reproduction. But so far, to the best of our knowledge, there is hardly any literature describing discordance among AMH and AFC and their clinical implications .Our study is first of its kind to demonstrate the concordant AMH values according to outcome of IVF .We can not imply the same AMH cut off values to all the women with different AFC. Therefore it is relevant to find out the individual cut off values for different AFC groups, thereafter to correlate the ovarian response and IVF outcome.

Index study showed a significant proportion, amounting to 79.93%, of women undergoing IVF treatment at the centre were showing discordant between AMH and AFC as classified by their percentiles according to their IVF outcome .There was wide fluctuation of percentile values of concordance of AHM for each AFC group, which indicate that same AHM value for different AFC group women may not generate same result. Although there was linear correlation of number of M2 oocytes and good quality embryos with AMH values but IVF outcome did not follow the same trend. When IVF outcome (pregnancy yes and no) was plotted over various AMH intervals as histogram, we found highest pregnancy rate in all concordant groups. Low and high AMH values in all AFC categories were associated with poorer pregnancy rate. For better IVF outcome, there should be a correlation between AFC and AMH. Though this is retrospective data analysis, strength lies in the fact that discordant value of AMH for every AFC group was described. This is in contrast to study by Raymond Li et al. [10] in which the same AMH range was generalised for the whole study group [10].

Conclusion

Current study described AMH range for each AFC category where best IVF results were obtained for our centre. Highest pregnancy rates were observed in all three AFC categories showing concordance between AFC and AMH values. Low and high AMH values in all AFC categories were associated with poorer pregnancy rate. M2 rate increased linearly with rise of AMH values in each category. Despite higher M2 rates reported with higher AMH values in each AFC category, lower pregnancy rates were reported. Women with negative pregnancy results showed significantly low numbers of M2 oocytes when compared with positive pregnancy within each AFC and AMH category. We hypothesised that discordance between the two may have adverse impact on outcome of IVF. Accumulation of more data is needed to further validate our study.

[lopezdorothy70-blog]

Effect of dandelion extracts on the proliferation of ovarian granulosa cells and expression of hormone receptors.

PMID:  Chin Med J (Engl). 2018 Jul 20 ;131(14):1694-1701. PMID: 29998889 Abstract Title:  Effect of Dandelion Extracts on the Proliferation of Ovarian Granulosa Cells and Expression of Hormone Receptors. Abstract:  Background: In the current society, infertility related to age has become a social problem. The in vitro fertilization (IVF) success rate in women with poor ovarian response (POR) is very low. Dandelion extract T-1 (DE-T1) is an effective component of the extract from the leaves and stems of Taraxacum officinale, which is one of the medicines used in some patients with POR, but its molecular mechanism remains unclear.Methods: Following IVF, ovarian granulosa cells (GCs) of sixty patients were extracted and divided into normal ovarian response (NOR) and POR groups. GCs were cultured in a dose-dependent and time-dependent manner with DE-T1, proliferation of GCs was determined by Cell Counting Kit-8 assay, and mRNA levels of insulin-like growth factor 1 receptor (IGF-1R), luteotropic hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), LHR, and CYP19A1 (aromatase) were determined by quantitative polymerase chain reaction. Progesterone and estradiol (E2) concentrations were determined by enzyme-linked immunosorbent assay.Results: The cell viability gradually increased with the progressive increase in the DE-T1 concentration. Compared with the control group (without DE-T1), the mRNA expressions of FSHR, LHR, IGF-1R, and CYP19A1 were upregulated after the addition of DE-T1, especially in the 2.5% DE-T1 group (P

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[abbkinesci-blog]

New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research

Interests on http://www.abbkine.com/assay-kitelisa-kit-3/

Hormones ELISA Kits from Abbkine Scientific

Abbkine Scientific newly debuts EliKine™ series of Hormones ELISA Kits, these kits including Testosterone ELISA Kit, Progesterone ELISA Kit, Estradiol ELISA Kit, fT3 ELISA Kit, fT4 ELISA Kit, T3 ELISA Kit, T4 ELISA Kit. They all developed as Competitive ELISA (quantitative) and Colorimetric m.... Click for moreHormones ELISA Kits from Abbkine Scientific.

[abbkine]

New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research

Hormones ELISA Kits from Abbkine Scientific

Abbkine Scientific newly debuts EliKine™ series of Hormones ELISA Kits, these kits including Testosterone ELISA Kit, Progesterone ELISA Kit, Estradiol ELISA Kit, fT3 ELISA Kit, fT4 ELISA Kit, T3 ELISA Kit, T4 ELISA Kit. They all developed as Competitive ELISA (quantitative) and Colorimetric measurement, directly from Serum, Plasma and Other biological fluids.

A hormone is any member of a class of signaling molecules produced by glands in multicellular organisms that are transported by the circulatory system to target distant organs to regulate physiology and behaviour. Hormones have diverse chemical structures, mainly of 3 classes are eicosanoids, steroids, and amino acid/protein derivatives. Hormones are used to communicate between organs and tissues for physiological regulation and behavioral activities, such as digestion, metabolism, tissue function.

Abbkine’s competitive Hormones ELISA Kits employ the competitive inhibition enzyme immunoassay technique with featured and specific detection antibodies. Exclusive EliKine™ streptavidin-HRP conjugate, and HRP substrate with other optimal components make EliKine portfolio be one of the best and most economical choices for ELISA assay researchers. Our complete, ready-to-use ELISA Kits reduce assay time and variability and are available in either 1 or 10 pre-coated plate options.

These featured EliKine™ Hormones ELISA Kits are suitable for Universal Reactivity and with a working time of 3-4 hours,  they also have following advantages:

● High efficiency, sensibility and specificity ● Optimized proposal for high repeatability, more stable ● 48 test pre-coated package for easy entrance ● Strip microplate formate with greater flexibility ● Suitable for multiple types of samples ● Professional techinical support and after-sales service

Below is EliKine™ Testosterone ELISA Kit Standard Curve.

                                                 Learn more about Hormones ELISA Kits, please click

https://www.abbkine.com/shop/?t=filter&cats%5B%5D=28&_assay%5B%5D=9024.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. is a leading biotechnology company that focuses on developing and providing innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.  Find more details, please visit the website at https://www.abbkine.com.

[calbiotechinc]

How Progesterone Assay Kits are Revolutionizing Veterinary Reproductive Studies

Reproductive health is a crucial aspect of veterinary science, particularly for livestock and companion animals. Efficient breeding management and early detection of reproductive issues are vital for animal health, productivity, and welfare. To read more, Click Here.

[abbkineus]

New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research

New Post has been published on http://www.abbkine.com/assay-kitelisa-kit-3/

Hormones ELISA Kits from Abbkine Scientific

Abbkine Scientific newly debuts EliKine™ series of Hormones ELISA Kits, these kits including Testosterone ELISA Kit, Progesterone ELISA Kit, Estradiol ELISA Kit, fT3 ELISA Kit, fT4 ELISA Kit, T3 ELISA Kit, T4 ELISA Kit. They all developed as Competitive ELISA (quantitative) and Colorimetric measurement, directly from Serum, Plasma and Other biological fluids.

A hormone is any member of a class of signaling molecules produced by glands in multicellular organisms that are transported by the circulatory system to target distant organs to regulate physiology and behaviour. Hormones have diverse chemical structures, mainly of 3 classes are eicosanoids, steroids, and amino acid/protein derivatives. Hormones are used to communicate between organs and tissues for physiological regulation and behavioral activities, such as digestion, metabolism, tissue function.

Abbkine’s competitive Hormones ELISA Kits employ the competitive inhibition enzyme immunoassay technique with featured and specific detection antibodies. Exclusive EliKine™ streptavidin-HRP conjugate, and HRP substrate with other optimal components make EliKine portfolio be one of the best and most economical choices for ELISA assay researchers. Our complete, ready-to-use ELISA Kits reduce assay time and variability and are available in either 1 or 10 pre-coated plate options.

These featured EliKine™ Hormones ELISA Kits are suitable for Universal Reactivity and with a working time of 3-4 hours,  they also have following advantages:

● High efficiency, sensibility and specificity ● Optimized proposal for high repeatability, more stable ● 48 test pre-coated package for easy entrance ● Strip microplate formate with greater flexibility ● Suitable for multiple types of samples ● Professional techinical support and after-sales service

Below is EliKine™ Testosterone ELISA Kit Standard Curve.

                                                 Learn more about Hormones ELISA Kits, please click

https://www.abbkine.com/shop/?t=filter&cats%5B%5D=28&_assay%5B%5D=9024.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. is a leading biotechnology company that focuses on developing and providing innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.  Find more details, please visit the website at https://www.abbkine.com.

[marketresearchandreports-blog]

Endocrine Testing Market To Gain From Rising Geriatric Population And Persisting Cases Of Illnesses Such As Diabetes And Obesity Till 2024

The global endocrine testing market is expected to reach USD 13.0 billion by 2024, according to a new report by Grand View Research, Inc.The growing geriatric population and persisting cases of illnesses, such as diabetes and obesity, are estimated to drive the market.

The target population for this industry is geriatric patients as they are highly prone to various chronic disorders. They have decreased immunity, are prone to age-related deformities, and are at a higher risk of developing complications. As per the WHO statistics on geriatric population, there would be a two-fold rise in the geriatric population; for example, in the U.S. by 2050, geriatric patient count of age 65 and above would be 83.7 million as compared to 43.1 million in 2012.

Sedentary lifestyles of people with unhealthy food habits, and increasing stress levels resulted in an increase in the diabetic and obese population. This demographic is highly susceptible to endocrine diseases and is anticipated to contribute to industry growth substantially.

Technological advancements in terms of accuracy and affordability resulted in an increased usage of these tests. For instance, the influx of test kits, for home-based testing has fostered the demand for these techniques, helping the market to grow.

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Further key findings from the study suggest:

·         The thyroid stimulating hormone test segment held a lucrative share of over 25.0% in 2015 among test type segments.The increasing cases of hyperthyroidism and hypothyroidism are anticipated to contribute to the growth.

·         In the technology segment, the immunoassay technology segment shared the highest market share owing to the increased analysis of hormonal levels using Radioimmunoassay(RIA) and enzyme-linked assays

·         The commercial laboratories in the end-use segment exhibited remunerative growth in 2015 and this can be attributed to the presence of sophisticated work environment, which yields faster and accurate diagnosis. Moreover, the dominance of private healthcare sector in developing nations contributed to the growth. For instance, in India, approximately 87.0% healthcare is privately financed. In 20 developing countries, their 40-70% GDP is utilized for out-of-pocket expenses in healthcare.

·         On the other hand, the home-based test segment is anticipated to witness a lucrative growth over the forecast period. Convenience and affordability of home-based testing are anticipated to contribute to the growth. Additionally, as per the U.S. Bureau of Labor Statistics, since easy-to-use diagnostic kits have been introduced the rank of home-based tests is expected to increase, which in turn isfurther expected to support the lucrative growth rate.

·         North America dominated the overall market with a revenue share of over 38.0% in 2015, owing to the presence of well-developed hospitals, reimbursement network, government funding, and the increasing health awareness among people

·         However, Asia Pacific is anticipated to be the fastest growing region with a CAGR of about 9.0% over the forecast period.Economic developments and the advancing healthcare system with the aid of supportive government initiatives are anticipated to assist the growth.

·         Key players of the industry include Abbott Laboratories, AB Sciex, Agilent Technologies, Biomedical Technologies, bioMérieuxSA, Bio-Rad Laboratories, DiaSorin, Hoffmann-La Roche Ltd., LabCorp, and Quest Technology.

·         Most of these companies have been in the business for over a decade and are bringing in expertise and technological advancements that are helping them sustain themselves in the competition.

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Grand View Research has segmented the endocrine testing market on the basis of test types, technology, end-use, and region.

  Global Endocrine Testing Market, By Test Types (Revenue, USD Million, 2013 - 2024)

·         Estradiol (E2) test

·         Follicle Stimulating Hormone (FSH) test

·         Human Chorionic Gonadotropin (hCG) Hormone test

·         Luteinizing Hormone (LH) Test

·         Dehydroepiandrosterone sulfate (DHEAS) test

·         Progesterone test

·         Testosterone test

·         Thyroid Stimulating Hormone (TSH) test

·         Prolactin test

·         Cortisol test

·         Insulin test

·         Others

  Global Endocrine Testing Market, By Technology (Revenue, USD Million, 2013 - 2024)

·         Tandem Mass Spectrometry

·         Immunoassay Technology

·         Monoclonal and Polyclonal Antibody Technology

·         Sensors Technology

·         Clinical Chemistry Technology

·         Other

  Global Endocrine Testing Market, By End-use (Revenue, USD Million, 2013 - 2024)

·         Hospitals

·         Commercial Laboratories

·         Ambulatory Care Centers

·         Home-based tests

·         Other settings

  Global Endocrine Testing Market, By Region (Revenue, USD Million, 2013 - 2024)

·         North America

o    U.S.

o    Canada

·         Europe

o    U.K.

o    Germany

·         Asia Pacific

o    Japan

o    China

·         Latin America

o    Brazil

o    Mexico

·         MEA

o    South Africa

o    Saudi Arabia

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About Grand View Research

Grand View Research, Inc. is a U.S. based market research and consulting company, registered in the State of California and headquartered in San Francisco. The company provides syndicated research reports, customized research reports, and consulting services. To help clients make informed business decisions, the company offers market intelligence studies ensuring relevant and fact-based research across a range of industries including technology, chemicals, materials, healthcare and energy.

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